WebShuttle Vector Systems Plasmid vectors using puromycin resistance as a selective marker have been developed for many methanogenic Archaea. The current vectors are constructed either with autonomous replication from rolling-circle origins, or else with the ability to integrate into the chromosome using cassette markers flanked by chromosomal DNA … WebDec 14, 2012 · YAC's or Yeast artificial chromosomes are shuttle-vectors, ( a shuttle vector is one that is able to replicate in other than one host organisms or 2 dissimilar cell types that is a prokaryotic and a eukaryotic cell) that have the ability to amplify in bacteria and can be utilized for the manipulation and cloning of huge DNA inserts in Saccharomyces …
Shuttle vector - Wikipedia
Webshuttle vector: a vector that contains both bacterial and eukaryotic replication signals; thus, replication can occur in both types of cells. See also: vector (4). WebMar 18, 1997 · New methods that allow, for the first time, genetic analysis in Archaea of the genus Methanosarcina are presented. First, several autonomously replicating plasmid shuttle vectors have been constructed based on the naturally occurring plasmid pC2A from Methanosarcina acetivorans.These vectors replicate in 9 of 11 Methanosarcina strains … poly gel led lamp set
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WebChoose pAdEasy-1 vector or any of these shuttle vectors, pShuttle, pShuttle-CMV, or pShuttle-CMV-lacZ vector, to clone DNA of interest with the AdEasy adenoviral vector system. Transformants are selected for kanamycin resistance, and recombinants are subsequently identified by restriction digestion. Once a recombinant is identified, it is … WebThis is one of a series of pBluescript-based YC-type (centromeric) yeast shuttle vectors (ATCC 77142-77145) differing in the yeast selectable marker gene. It encodes the beta galactosidase alpha peptide for blue-white color detection of inserts and also has the T7 and T3 promoters for in vitro RNA synthesis and priming sites for sequencing. It is useful in … WebJul 22, 2024 · Transiently replicating shuttle vectors that need to be recognized by large T antigen in order to replicate in human cells. Episomal shuttle vectors work to establish … polygel made in the usa