WebIn CHX-chase analysis, the administration of a global protein translation inhibitor (such as cycloheximide, CHX) to block protein translation is performed (CHX-pulse phase). This is followed by examining the reduction in the levels of a target protein ‘chase’ via sodium dodecyl sulfate–polyacrylamide gel electrophoresis and immunoblotting. WebAug 1, 2015 · Endogenous DISC1 protein expression in the total protein extract was gradually decreased with increased chase time points (from 3 to 24 h) following a characteristic exponential decay in the cells treated with CHX at a concentration of 20 μg/ml, which inhibits protein synthesis.

Analysis of Protein Stability by the Cycloheximide Chase Assay

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Cycloheximide - an overview ScienceDirect Topics

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